Apex MM
Apex MM SEC columns
Easily isolate pure, functional EVs with high yield without the lipoproteins
Apex MM for peak performance
2nd generation EV isolation
- Highest purity for proteomics and genomics applications
- Reproducible performance <10% CV
- Sample volume 0.1-1.0 mL
- Validation in cell culture plasma, serum, urine and CSF
- Combines SEC and multi-mode resin for superior purity
Apex mini
Apex MM for lipoprotein depletion
More than just SEC
By combining SEC with a multi-mode (MM) resin that uses hydrophobic interaction and ion-exchange to selectively bind and remove lipoproteins and other soluble contaminants, Apex MM columns significantly enhancing EV purity.
Apex MM performance with plasma
Apex MM: Optimized for maximum EV purity in plasma/serum samples—optimal for unbiased assays like mass spec and flow cytometry.
| Column Type | Yield | Purity (HSA) | Purity (ApoA1) | Purity (ApoB-100) | Pore-Size |
| Apex MM | 1.5 | 2.2 | 1.1 | 1.8 | <50 nm |
| Competitor | 1.0 | 1.0 | 1.0 | 1.0 | 70 nm |
Definitions:
-
Yield = EVs in fractions 1–3, normalized to Competitor
-
Purity (HSA) = Yield/HSA, normalized to Competitor
-
Purity (ApoA1) = Yield/ApoA1, normalized to Competitor
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Purity (ApoB-100) = Yield/ApoB-100, normalized to Competitor
EV profiles from 0.5 mL plasma, as measured by Atlas ELISA, after SEC with Apex MM columns and qEV70.
Apex mini, smaller size, same performance
50% more EVs and 2x purity
- Optimized for small sample volumes (0.1 – 0.3 mL)
- Similar performance to Apex MM
- Easy-open cap
- Works on the Ascent instrument
Same technology, smaller size. The Apex MM (0.5 mL) and Apex MM (0.15 mL) have similar yields and purities.
Plasma Sample
NTA
ONI
Technical information
The Apex MM column purifies extracellular vesicles (EVs) from biological fluids such as plasma, serum, urine, cell culture media, or cerebrospinal fluid (CSF).
Everest Biolabs Atlas EV and human serum albumin (HSA) ELISA kits can be used to optimize EV yield and purity during fraction collection.
| Column format | Column | mini |
|---|---|---|
| Column type | MM / 4B / 6B | MM |
| Input sample volume | 0.5 - 1.0 mL | 0.1 - 0.3 mL |
| Column volume | 8.75 mL | 3.5 mL |
| Column reproducibility | 10% CV | |
| Sample types | plasma, serum, urine, CSF, cell culture media | |
| Resin types | cross-linked agarose plus multi-mode | |
Discuss your isolation needs with our scientific team
Latest Apex Application Notes
Can SEC be a scalable and more reproducible alternative to ultracentrifugation?
Can SEC be a scalable and more reproducible alternative to ultracentrifugation?
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Advancing Plasma Derived Extracellular Vesicle Analysis Through High-Purity Isolation and Particle-Specific Quantification
Advancing Plasma Derived Extracellular Vesicle Analysis Through High-Purity Isolation and Particle-Specific Quantification
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Drip speed matters: Enhance EV isolation reproducibility with Apex columns and validate using the Atlas ELISA
Drip speed matters: Enhance EV isolation reproducibility with Apex columns and validate using the Atlas ELISA
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Optimizing Extracellular Vesicle Isolation: Why Apex MM Columns Outperform Conventional SEC Columns for isolating EVs from Plasma or Serum
Optimizing Extracellular Vesicle Isolation: Why Apex MM Columns Outperform Conventional SEC Columns for isolating EVs from Plasma or Serum
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Why is SEC becoming the most widely used EV isolation technique?
