Apex SEC Columns
The right SEC column for every sample
High-yield, high-purity isolation of intact, functional EVs, a range of size-exclusion chromatography columns matched to your biofluid and assay.
- Reproducible performance <10% CV
- Sample volume 0.1–1.0 mL
- Available with SEC and multi-mode resins
- Validated in cell culture, plasma, urine, serum & CSF
- Compatible with Ascent and Summit instruments
Why size-exclusion chromatography
Yield. Purity. Integrity. Other EV isolation methods make you trade one for another, SEC delivers all three at once, which is why it’s become the standard.
>90%
High EV yield
Recover the majority of your vesicles.
>99.9%
High purity
Depletion of the major protein contaminants in plasma, albumin and lipoproteins.
Intact & functional
Vesicle morphology confirmed by TEM and peer-reviewed studies.
Apex SEC working principle
SEC separates EVs from smaller contaminants by size, vesicles elute first, small proteins later. Pore size sets the trade-off: smaller pores favor yield, larger pores favor purity. Apex offers both, plus a multi-mode option for the hardest separations.
Apex SEC Columns & Plates
Isolation that matches your purity and throughput needs
Size-exclusion chromatography isolates extracellular vesicles gently and reproducibly. Choose the Apex column matched to your sample: from highest yield to peak lipoprotein purity.
- Same resin and bed volume in column and 24-well plate, results transfer directly
- Seamlessly scale from a single column to a 24-well plate
- Mini format for precious 0.1–0.3 mL inputs
- Run manually, or automate on Ascent (columns) and Summit (plates & columns)
Apex mini
0.1–0.3 mL
Apex column
0.5–1.0 mL
Apex 24-well plate
24 samples in parallel
One Apex family, from maximum yield to maximum purity. The more complex the sample, the more purifying power you need.
Choose your column
Match the Apex column to your sample and downstream assay: from highest yield to peak lipoprotein purity.
| Apex 6BLearn more → | Apex 4BLearn more → | Apex MMLearn more → | |
|---|---|---|---|
| Optimized for | Highest yield | High purity vs. free proteins | High purity vs. lipoproteins |
| Yield | >90% | >70% | >50% |
| Human serum albumin depletion | >99.9% | >99.99% | >99.999% |
| ApoB (LDL) depletion | 40.0% | >80.0% | >98.0% |
| ApoA1 (HDL) depletion | >99% | >99.9% | >99.99% |
| Pore size / resin | 20 nm | 35 nm | Multi-mode |
| Best for | Low-complexity samples (serum-free CCM, urine, CSF) | Complex biofluids | Plasma & serum |
Column selector
Find the right column for your workflow
| Sample type | Analysis | Recommended column |
|---|---|---|
| Plasma / serum / CCM with FBS (lipoprotein-rich) | Label-free single-particle count (NTA / nanoflow) | Apex MM |
| Untargeted (mass spec / proteomics) | Apex MM | |
| Marker-based (ELISA / WB / single particle) | Apex 4B | |
| Serum-free CCM, CSF, urine (low-complexity) | Most applications | Apex 6B |
Technical specifications
Specifications & formats
| Specification | Apex 6BLearn more → | Apex 4BLearn more → | Apex MMLearn more → |
|---|---|---|---|
| Resin / pore size | 20 nm SEC | 35 nm SEC | SEC + multi-mode |
| Bed volume | 9.0 mL · mini 3.3 mL | ||
| Sample volume | 0.5–1.0 mL · mini 0.1–0.3 mL | ||
| Formats | Single column, mini, 24-well plate | Single column, mini, 24-well plate | Single column, mini |
| Compatible instruments | Ascent (columns) · Summit (plates & columns) | ||
| Validated biofluids | Cell culture, plasma, serum, urine, CSF | ||
Reproducible by design
Apex columns are optimized for consistent drip speed, producing a reproducible EV elution profile. Pairing with Ascent and Summit further minimizes variability across columns.
<10%
CV in EV yield
Intact & functional EVs
SEC isolates vesicles gently, preserving their structure and biological activity. Vesicle morphology is confirmed by transmission electron microscopy, and SEC-isolated EVs underpin a growing body of peer-reviewed work.
One workflow, end to end
Isolate, automate, and quantify, the complete EV workflow, built to work together.
Contact us
Match Apex to your applications
Tell us about your biofluid and downstream assay, and our scientists will help you match the right column to your workflow and share a protocol to get you started.