Absorbance ELISA – Simple and reliable EV yield and purity assessment using standard plate readers.
Chemiluminescence (Lumi) ELISA – High sensitivity, wide dynamic range, and customizable capture (Homebrew options) for profiling disease-relevant markers.
| Atlas Human EV | Atlas HSA | Atlas Lumi Human EV | Atlas Lumi Homebrew | |
| Dynamic range | 2 logs | 2 logs | 4 logs | 4 logs |
| Limit of detection | 1e8 | 1e8 | 1e7 | 1e7 |
| Reader compatibility | Absorbance | Absorbance | Chemiluminescence | Chemiluminescence |
| Custom targets | ✔ |
LOD ~ 1e7 EVs/mL
Dynamic Range 4+ logs
This kit contains enough reagents for 96 wells.
| Absorbance | Chemiluminescence (Lumi) | |
| Reactive species | Human | Human |
| Well format | 8 x 12 strips | 8 x 12 strips |
| Dynamic range | 1e8 – 1e10 particles / mL | 1e7 - 1e11 particles / mL |
| Assay reproducibility (CV) | 5.5% | 5.75% |
| Limit of detection (LOD) | 1e8 particles / mL | 1e7 particles / mL |
| Lower limit of quantification (LLOQ) | 3.5e8 particles / mL | 1.4e7 particles / mL |
| Sample types | Plasma Serum Urine CSF Cell culture media | Plasma Serum Urine CSF Cell culture media |
The Atlas Human EV ELISA has one 3-hour incubation and one wash step. The total hands-on time is ~15 minutes for plating and washing.
The Atlas Human EV ELISA uses antibodies against the tetraspanins CD9, CD63, and CD81. These are used as both capture and detection antibodies.
The average LOD for the Atlas EV Human ELISA is < 1e8 EVs/mL. For the Atlas Lumi Human EV ELISA the average LOD is < 1e7 EVs/mL.
Yes. The ELISA has been validated in plasma, serum, urine, cell culture media, and cerebrospinal fluid (CSF). Data for these biofluids is available on the datasheet.
No. The Atlas Human EV ELISA kit has 12 strips of 8 wells. You can run individual strips or the full 96-well plate.
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