Application Notes Archives | Everest Biolabs

Mar 23

Can SEC be a scalable and more reproducible alternative to ultracentrifugation?

By admin Apex, Application Notes

The shift toward gentler isolation EV labs are moving away from the rotor toward Size Exclusion Chromatography (SEC). The reason: SEC preserves EV biophysical integrity and functionality more effectively than…

Jan 12

Ascent-coupled ultrafiltration enables concentrated EV recovery after SEC with Apex columns

By admin Application Notes, Ascent

Size exclusion chromatography (SEC) is widely used to isolate extracellular vesicles (EVs) from plasma; however, the EV-containing fractions obtained are often dilute, which can limit downstream analyses requiring low input…

Dec 02

When Every Fraction Counts: How the Ascent Platform Transforms EV Isolation

By admin Application Notes, Ascent

The Hidden Bottleneck in EV Research For scientists working with extracellular vesicles (EVs), the promise of discovery is often slowed by the realities of the bench. EV isolation, especially via…

Dec 02

Advancing Plasma Derived Extracellular Vesicle Analysis Through High-Purity Isolation and Particle-Specific Quantification

By admin Apex, Application Notes, Atlas

AuthorsTal Gilboa, Ph.D.*, Dr. Christina Klasen**, Dr. Lena Ball** A collaboration of Introduction Everest Biolabs and Particle Matrix have partnered to establish a unified workflow for extracellular vesicle (EV) analysis,…

Oct 10

Atlas EV ELISA: A scalable solution for Extracellular Vesicle quantification

By admin Application Notes, Atlas

Quantifying extracellular vesicles (EVs) is essential for optimizing isolation workflows, developing EV-based assays, and advancing biomarker discovery. Single particle techniques such as flow cytometry and nanoparticle tracking analysis (NTA) face…

Aug 18

Drip speed matters: Enhance EV isolation reproducibility with Apex columns and validate using the Atlas ELISA

By admin Apex, Application Notes

Column-to-column variability in extracellular vesicle (EV) isolation using size exclusion chromatography (SEC) can significantly impact downstream results—shifting the EV elution profile and compromising reproducibility. Here, we explore how drip speed…

Jun 11

Optimizing Extracellular Vesicle Isolation: Why Apex MM Columns Outperform Conventional SEC Columns for isolating EVs from Plasma or Serum

By admin Apex, Application Notes

IntroductionExtracellular vesicles (EVs) are essential to diagnostics and therapeutic research, serving as rich sources of biomarkers and key mediators of intercellular communication. Size Exclusion Chromatography (SEC) is widely used for isolating…

Jun 04

Automation (for) the Lab People: How Everest Biolabs is Revolutionizing EV Isolation with SEC Automation

By admin Application Notes, Ascent, Summit

Growth of SEC EV isolation driven by innovation at Everest Biolabs. Over the past two decades, extracellular vesicles (EVs) have transitioned from being considered cellular debris to taking center stage…

Feb 03

Virtual demonstration of the Ascent instrument for automated EV isolation by size exclusion chromatography

By admin Application Notes

https://www.youtube.com/watch?v=-Gw5sIPjwPo Everest Biolabs presents a virtual demonstration of the Ascent instrument, an advanced system for automated extracellular vesicle (EV) isolation using size exclusion chromatography (SEC).

Dec 05

Why is SEC becoming the most widely used EV isolation technique?

By admin Apex, Application Notes

Size Exclusion Chromatography (SEC) is a commonly used method for extracellular vesicle (EV) and exosome isolation from biological fluids. SEC is easy, reproducible, and provides a high yield of purified…

Application Notes10

Can SEC be a scalable and more reproducible alternative to ultracentrifugation?

Ascent-coupled ultrafiltration enables concentrated EV recovery after SEC with Apex columns

When Every Fraction Counts: How the Ascent Platform Transforms EV Isolation

Advancing Plasma Derived Extracellular Vesicle Analysis Through High-Purity Isolation and Particle-Specific Quantification

Atlas EV ELISA: A scalable solution for Extracellular Vesicle quantification

Drip speed matters: Enhance EV isolation reproducibility with Apex columns and validate using the Atlas ELISA

Optimizing Extracellular Vesicle Isolation: Why Apex MM Columns Outperform Conventional SEC Columns for isolating EVs from Plasma or Serum

Automation (for) the Lab People: How Everest Biolabs is Revolutionizing EV Isolation with SEC Automation

Virtual demonstration of the Ascent instrument for automated EV isolation by size exclusion chromatography

Why is SEC becoming the most widely used EV isolation technique?

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