Apex 4B
Apex 4B and 6B SEC columns
Easily isolate pure, functional EVs with high yield
Apex SEC columns and Plates
Isolation that matches your purity and throughout needs
- Reproducible performance <10% CV
- Sample volume 0.5-1.0mL
- Available in single column and plate format
- Validated in Cell culture, Plasma, Serum, Urine and CSF
Apex 6B
Apex 4B
Apex 6B
EV profiles from 0.5 mL plasma, as measured by Atlas ELISA, after SEC with Apex 4B and 6B (solid lines), and Human Serum Albumin measure in each fraction (dashed lines)
Apex 4B and 6B performance
- Apex 4B
- Optimized for high purity and lowest protein co-elution
- ~5X higher purity against secreted proteins
- Apex 6B
- Optimized for highest EV yield
- ~20% more EVs
Apex 4B and 6B maintains performance with reuse
Apex maintains performance and drip speed after 5 reuses. Columns were tested by running 0.5 mL of human plasma sample, and pooling fractions 1–3, which represent the EV fractions.
Columns were washed with 4 mL of NaOH, followed by 17 mL of 1X PBS between reuses. EVs and HSA were quantified using the Atlas ELISA
Apex 4B and 6B isolate the same EVs
EV composition and size comparison. Super resolution imaging (ONI) and size comparison by NTA and TEM show that Apex 4B and 6B purify EVs with the same tetraspanin composition and size.
Cell culture Sample
NTA/TEM
ONI
Plasma Sample
NTA/TEM
ONI
Technical information
The Apex size exclusion column (SEC) purifies extracellular vesicles (EVs) from biological fluids such as plasma, serum, urine, cell culture media, or cerebrospinal fluid (CSF).
There are Apex columns for your different samples types and volumes.
| Column format | Column | Plate | mini |
|---|---|---|---|
| Column type | MM / 4B / 6B | 4B / 6B | MM |
| Input sample volume | 0.5 - 1.0 mL | 0.5 - 1.0 mL | 0.1 - 0.3 mL |
| Column volume | 9.0 mL | 9.0 mL | 3.0 mL |
| Column reproducibility | 10% CV | ||
| Sample types | plasma, serum, urine, CSF, cell culture media | ||
| Resin types | 4% or 6 % cross linked agarose beads, cross linked agarose plus multi-mode | ||
| Exclusion limit | 35 nm or 20 nm | ||
Discuss your isolation needs with our scientific team
Latest Apex Application Notes
Can SEC be a scalable and more reproducible alternative to ultracentrifugation?
Can SEC be a scalable and more reproducible alternative to ultracentrifugation?
Read More
Advancing Plasma-Derived Extracellular Vesicle Analysis Through High-Purity Isolation and Particle-Specific Quantification
Advancing Plasma-Derived Extracellular Vesicle Analysis Through High-Purity Isolation and Particle-Specific Quantification
Read More
Drip speed matters: Enhance EV isolation reproducibility with Apex columns and validate using the Atlas ELISA
Drip speed matters: Enhance EV isolation reproducibility with Apex columns and validate using the Atlas ELISA
Read More
Optimizing extracellular vesicle isolation: Why Apex MM columns outperform conventional SEC columns for isolating EVs from plasma or serum
Optimizing extracellular vesicle isolation: Why Apex MM columns outperform conventional SEC columns for isolating EVs from plasma or serum
Read More
Why is SEC becoming the most widely used EV isolation technique?
Why is SEC becoming the most widely used EV isolation technique?
Read MoreFrequently Asked Questions
How many times can Apex columns be used?
We have tested Apex 4B/6B columns for up to 5 uses.
What is the repeatability of isolation after regeneration cycles?
Apex columns have a 5% CV in EV yield for up to 5 uses when measured with the Atlas EV ELISA. We know how important repeatability is, so we developed a tightly controlled process for manufacturing columns to ensure the most repeatable columns.
Should I use Apex MM or Apex 6B or Apex 4B?
Apex MM columns are optimized for maximum EV purity in plasma/serum samples. These are ideal for unbiased assays like Mass Spec and flow cytometry. Apex 6B are optimized for the highest total EV recovery. These are ideal for targeted downstream analysis, such as immunoassays, label-based imaging assays, label-based flow assays, western blots, and pull-down using a specific surface marker. Apex 4B are optimized for the highest purity. These are ideal for unbiased downstream analysis, including proteomics and RNA-seq.
Does the entire plate need to be used at one time?
No, the plate seal can be cut to use only 4 wells at one time and the Summit instrument allows skipping used wells so you can maximize the utility of each and every Apex plate.
