Summit Instrument

The Summit instrument can process from 4 to 48 samples at once. Simply load your samples, choose your parameters, and press run. That’s it! Your isolated EV’s will be loaded into a 96-well plate and ready for analysis when you get back.

A typical isolation takes 1-2 hours. However, the time varies depending on how many samples you run, the type of biofluid, and whether you concentrate the isolated EV’s. Luckily, it’s completely automated, so you can walk away and get other work done while it’s running.

The Summit can collect 1-3 fractions when you process up to 24 samples. If you are processing 48 samples, it collects 1 fraction per sample. The fraction volume can be configured from 0.5-2.0mL to maximize your EV yield.

The Summit uses ultrafiltration (UF) to concentrate the isolated EV’s. The fractions are collected directly into the 24-well UF plate and vacuum is applied. The Summit monitors the sample volume using an ultrasonic sensor and stops it when the sample is fully concentrated. It checks the volume again and refills each well with 100µLof new buffer. The UF plate is gently rocked to make sure your EVs aren’t stuck to the filter before the sample is pipetted into your output plate.

The Summit UF plates come with polyether sulfone (PES) membrane specifically modified to minimize protein and nucleic acid binding. They are available in 10, 30, 50, and 100 kDa MWCOs. The Summit UF plates will also be available with a regenerated cellulose membrane (10 kDa MWCO). These offer >90% recovery with certain biofluids to make sure your EVs stay put.

Transferring from the Ascent to the Summit is a piece of cake. Just choose the same parameters that you dialed in on the Ascent and your elution profile will be identical!

The Summit SEC Plate uses the same resin type and bed volume as the Apex Columns. We know how important reproducibility is, so we put a ton of effort into making sure the Apex Columns and SEC Plates work the same every time. You can switch back and forth between them and rest assured that your results will be the same.

The Summit can run any Apex column using the Apex Column adapter.

This depends on how many samples you want to isolate, how many fractions you collect, and whether you concentrate your fractions. But fear not, Everest Biolabs has everything you need to isolate your samples your way. Get the Summit Snack Pack with everything you need to isolate and concentrate 24 samples, or choose the consumables a’ la carte and customize it to your needs.

The summit is approximately 28” x 26” x 30” (WxDxH) (700mm x 650mm x 750mm). It is designed to fit on a standard lab bench, so there is no need to make any special accommodation for it.