Atlas EV ELISA Kits
Simple 1-step intact EV quantification assay
Atlas ELISA Kits
Easy and reliable EV quantification assays
- Run direct from biofluid or SEC fractions
- Validated in plasma, serum, CSF, urine, cell culture media
- Only 50 μL of sample – no need to purify or concentrate
- Run 8 wells at a time or the full 96-well plate
The Atlas EV ELISA is available in two formats:
Absorbance ELISA – Simple and reliable EV yield and purity assessment using standard plate readers.
Chemiluminescence (Lumi) ELISA – High-sensitivity, high-dynamic range, and customizable capture (Homebrew options) for profiling disease-relevant markers.
| Atlas Human EV | Atlas Lumi Human EV | |
|---|---|---|
| Dynamic range | 2 logs | 4 logs |
| Limit of detection | 1e8 | 1e7 |
| Reader compatibility | Absorbance | Chemiluminescence |
| Custom target (Homebrew) option | ✓ |
Detect your specific EV target with Atlas Lumi Homebrew
Customizable capture, high-sensitivity, high-dynamic range ELISA for biomarker quantification
- Easy 1-step custom capture
- Requires 100 μg of antibody
Technical information
The Atlas Human EV ELISA is an immunoassay to measure intact extracellular vesicles (EVs) in plasma, serum, urine, cell culture media, or cerebrospinal fluid (CSF).
This kit contains enough reagents for 96 wells.
| Absorbance | Chemiluminescence (Lumi) | |
|---|---|---|
| Reactive species | Human | Human |
| Well format | 8 x 12 strips | 8 x 12 strips |
| Dynamic range | 1e8 – 1e10 particles / mL | 1e7 - 1e11 particles / mL |
| Assay reproducibility (CV) | <10% | <10% |
| Limit of detection (LOD) | 1e8 particles / mL | 1e7 particles / mL |
| Lower limit of quantification (LLOQ) | 3.5e8 particles / mL | 1.4e7 particles / mL |
| Sample types | Plasma Serum Urine CSF Cell culture media | Plasma Serum Urine CSF Cell culture media |
Discuss your analytic needs with our scientific team
How should I quantify my EVs from biofluids? Western Blot, ELISA, or single-particle counting
How should I quantify my EVs from biofluids? Western Blot, ELISA, or single-particle counting
Read More
Advancing Plasma-Derived Extracellular Vesicle Analysis Through High-Purity Isolation and Particle-Specific Quantification
Advancing Plasma-Derived Extracellular Vesicle Analysis Through High-Purity Isolation and Particle-Specific Quantification
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Atlas EV ELISA: A scalable solution for extracellular vesicle quantification
Atlas EV ELISA: A scalable solution for extracellular vesicle quantification
Read MoreFrequently Asked Questions
How long does it take to run a sample?
The Atlas Human EV ELISA has one 3-hour incubation and one wash step. The total hands-on time is ~15 minutes for plating and washing.
What are the capture and detection antibodies?
The Atlas Human EV ELISA uses antibodies against the tetraspanins CD9, CD63, and CD81. These are used as both capture and detection antibodies.
What is the detection limit of the Atlas Human EV ELISA?
The average LOD for the Atlas EV Human ELISA is < 1e8 EVs/mL. For the Atlas Lumi Human EV ELISA the average LOD is < 1e7 EVs/mL.
Can the Atlas Human EV ELISA be used to quantify EVs directly from biofluids?
Yes. The ELISA has been validated in plasma, serum, urine, cell culture media, and cerebrospinal fluid (CSF). Data for these biofluids is available on the datasheet.
Do you have to use the entire plate at once?
No. The Atlas Human EV ELISA kit has 12 strips of 8 wells. You can run individual strips or the full 96-well plate.
