Atlas ELISA Kits
Simple 1-step intact EV quantification
The Atlas EV ELISA detects and quantifies the tetraspanins CD9, CD63 and CD81 simultaneously on intact vesicles — run directly from biofluids or SEC fractions, using just 50 µL of sample.
- One-step assay against CD9, CD63 and CD81
- Just 50 µL — no purification or concentration needed
- Absorbance and chemiluminescence (Lumi) formats
- 12 × 8-well strips — run a few wells or a full plate
50 µL
Diluted sample per well
3-in-1
Detects intact EVs via pan-tetra
1-step
Single incubation protocol
96
Wells (12 × 8 strips)
How it works
Sample to signal in one incubation
Add sample and HRP-modified anti-CD9/CD63/CD81 antibodies, incubate, wash, add substrate, and read. Intact EVs are captured and detected in a single step — no lysis or purification required.
One-step capture and detection
The protocol
Minimum hands-on time
A single ~3-hour incubation and one wash — about 15 minutes of hands-on time from sample to readout.
Incubation
Add diluted sample and the antibody mix to the well plate and incubate (~3 hours).
Wash
Wash the wells 5× with Wash Buffer to remove unbound material.
Signal generation
Add 100 µL TMB substrate (5–10 min), then 100 µL Stop Solution.
Signal readout
Measure absorbance on a standard plate reader — or luminescence for the Lumi format.
Choose your format
Two formats, one assay
Choose Absorbance for robust, routine quantification on a standard plate reader, or Lumi chemiluminescence for sensitive detection with a wider dynamic range and customizable targets.
| Absorbance | Lumi (chemi) | |
|---|---|---|
| Readout | Colorimetric (absorbance) | Chemiluminescence |
| Instrument | Standard plate reader | Luminometer / imager |
| Dynamic range | 2 logs (10⁸–10¹⁰ /mL) | 4 logs (10⁷–10¹¹ /mL) |
| Limit of detection | 1×10⁸ EVs/mL | 1×10⁷ EVs/mL |
| Reproducibility (CV) | < 10% | < 10% |
| Custom capture (Homebrew) | — | Available |
| Best for | Routine yield & QC across cohorts | Sensitive, customizable detection |
Lumi extends the range
Custom targets
Detect your specific EV target with Atlas Lumi Homebrew
Customizable capture, high-sensitivity, high-dynamic-range ELISA for biomarker quantification.
- Easy 1-step custom capture
- Requires 100 µg of antibody
How Atlas Lumi Homebrew works
Technical information
Atlas EV ELISA specifications
| Specification | Atlas Human EV ELISA |
|---|---|
| Targets | CD9, CD63 and CD81 (tetraspanins) |
| Antibody role | Used as both capture and detection antibodies |
| Reactive species | Human EVs only; not validated for other species |
| Sample volume | 50 µL |
| Sample types | Plasma, serum, urine, CSF, cell culture media, or SEC fractions |
| Sample preparation | None — run direct, no purification or concentration |
| Well format | 8 × 12 strips (96-well); run strips or a full plate |
| Protocol | One-step incubation (~3 h), then wash and develop; ~15 min hands-on |
| Reproducibility | < 10% CV |
| Storage | 2–8 °C |
One workflow
Quantify what you isolate
Use Atlas to read out EV yield and quality from Apex SEC fractions, or directly from biofluids — the same assay that validates Ascent and Summit isolation runs.
Application notes
Latest Atlas application notes
FAQ
Frequently asked questions
What does the Atlas EV ELISA detect?
It uses antibodies against the tetraspanins CD9, CD63 and CD81 — detecting all three simultaneously, as both capture and detection antibodies — to quantify intact extracellular vesicles.
How long does the assay take?
There is a single ~3-hour incubation followed by one wash step, with only about 15 minutes of hands-on time. Then add substrate, stop, and read.
Do I need to purify my sample first?
No. The assay runs directly on biofluids (plasma, serum, urine, CSF, cell culture media) or on purified EVs such as SEC fractions, using only 50 µL — no purification or concentration required.
What is the limit of detection?
The average LOD is 1×10⁸ EVs/mL for the Absorbance ELISA and 1×10⁷ EVs/mL for the Lumi (chemiluminescence) ELISA, with assay reproducibility under 10% CV.
Can I detect a custom target?
Yes — the Atlas Lumi Homebrew format supports customizable capture antibodies for a one-step custom assay. Just provide 100 µg of your antibody.
Do I have to run the whole plate at once?
No. The kit has 12 strips of 8 wells, so you can run individual strips or scale up to the full 96-well plate.
Contact us
Quantify your EVs
Tell us your sample types and readout needs, and our scientists will recommend an Atlas format and share a datasheet to get you started.