You may adjust these values for your application to work with different column types, between 1 and 8 columns, wash volumes, number of fractions to collect, and the fraction volume.
Peak EV isolation
Ascent Instrument
Automated, high-throughput EV isolation with SEC columns
- Run up to 8 columns in parallel
- Collect up to 12 fractions per column
- Wash up to 8 columns
- Compatible with different column types
- Programmable fraction collection sequences
Programmable fraction collection
Optimize your fraction collection for your application.
Technical information
The Ascent instrument is an automated high-throughput fraction collector for isolation of extracellular vesicles (EVs) with SEC columns.
It can operate up to 8 columns simultaneously and is compatible with Apex columns as well as other brands. The programmable fraction collection allows you to optimize collection for any application.
| SEC column capability | Apex 4B Apex 6B Izon qEVoriginal Izon qEV1 Izon qEVsingle |
| Dimensions (WxDxH) | 397mm x 364mm x 331mm 15-5/8” x 14-5/8” x 13” |
| Weight | 13.6 Kg / 30.0 lbs. |
| Input Power | 100~240 VAC (50-60Hz) |
| Power Supply Unit | 24 Vdc, 2.1A, 50W |
| Environmental Temperature | 10°C -24°C (50°F-75°F) |
| Relative Humidity | 30-80% RH |
| Bottle Capacity | 1000mL |
| Connectivity | USB-C (for software updates) |
| Chemical Compatibility | PBS, DI, 70% ethanol, dilute bleach |
Frequently Asked Questions
How long does it take to purify samples on the Ascent?
Running 1 or 8 Apex columns takes about an hour. Since columns are run in parallel, it takes about the same time for any number of columns. First, the columns are washed to remove buffer containing antimicrobial agents. This step takes ~45 minutes. Then the fractions are collected. This takes ~5-15 minutes depending on how many fractions are collected. Apex 4B and 6B columns have the same run time.
Can the Ascent run columns from other suppliers?
Yes. In addition to the Apex columns, the Ascent is compatible with qEVoriginal, qEV1, and qEVsingle columns. If you would like support for additional columns, please contact us.
Can the fraction collection sequence on the Ascent be customized?
Yes. The fraction collection sequence on the Ascent is programmable. The user can adjust the discard volume and the fraction volume according to the input sample volume.
What is Column Saver?
Column Saver automatically keeps columns hydrated. This prevents your columns from drying out if you are not near the instrument when the wash or fraction collection completes. The Column Saver slowly drips buffer into the columns while the Ascent waits for an action from the user (adding samples or removing the columns when it completes).
How does the Ascent accurately collect the correct fraction volume?
Ascent Instrument
The Ascent has a high-precision liquid handling system combined with an advanced optical drop detection platform to precisely collect fractions. This approach is robust to vibrations and can be used on any lab bench.
