This kit contains enough reagents for 96 wells.
Peak EV analysis
Atlas ELISA Kits
Easy and reliable EV quantification and purity assays
- Run direct from biofluid or SEC fractions
- Only 50 μL of sample – no need to purify or concentrate
- Run 8 wells at a time or the full plate
- Human EV ELISA provides EV quantification
- HSA ELISA measures the purity of your sample
Technical information
The Atlas Human EV ELISA is an immunoassay to measure intact extracellular vesicles (EVs) in plasma, serum, urine, cell culture media, or cerebrospinal fluid (CSF).
| Reactive species | Human |
| Well format | 8 x 12 strips |
| Dynamic range | 1e8 – 1e10 particles / mL |
| Assay reproducibility (CV) | 5.5% |
| Limit of detection (LOD) | 1e8 particles / mL |
| Lower limit of quantification (LLOQ) | 3.5e8 particles / mL |
| Sample types | Plasma Serum Urine CSF Cell culture media |
Frequently Asked Questions
How long does it take to run a sample?
The Atlas Human EV ELISA has one 3-hour incubation and one wash step. The total hands-on time is ~15 minutes for plating and washing.
What are the capture and detection antibodies?
The Atlas Human EV ELISA uses antibodies against the tetraspanins CD9, CD63, and CD81. These are used as both capture and detection antibodies.
What is the detection limit of the Atlas Human EV ELISA?
The average LOD is < 1e8 EVs/mL.
Can the Atlas Human EV ELISA be used to quantify EVs directly from biofluids?
Yes. The ELISA has been validated in plasma, serum, urine, cell culture media, and cerebrospinal fluid (CSF). Data for these biofluids is available on the datasheet.
Do you have to use the entire plate at once?
No. The Atlas Human EV ELISA kit has 12 strips of 8 wells. You can run individual strips or the full 96-well plate.
