Size Exclusion Chromatography (SEC) is a commonly used method for extracellular vesicle (EV) and exosome isolation from biological fluids. SEC is easy, reproducible, and provides a high yield of purified EV’s that retain their functionality.
Peak EV isolation
Apex SEC Columns
High performance isolation
- Reproducible performance < 5% CV
- Available with two gel types: 4B (high-purity) & 6B (high-yield)
- Sample volume 0.5-1.0 mL
How do I apply Apex columns to my research?
Apex 6B Column: Optimized for the highest EV recovery. Ideal for targeted downstream analysis including immunoassays, label-based imaging assays, label-based flow assays, western blots, and pull-down using a specific surface marker.
Apex 4B Column: Optimized for the highest purity. Ideal for unbiased downstream analysis, including proteomics and RNA-seq.
For the best reproducibility and throughput, Apex columns combined with the Ascent instrument can elevate your research.
Apex performance with plasma
- Reproducible performance <5% CV
- Apex 4B ~20X higher purity
- Apex 6B ~20% more EVs
Technical information
The Apex size exclusion column (SEC) purifies extracellular vesicles (EVs) from biological fluids such as plasma, serum, urine, cell culture media, or cerebrospinal fluid (CSF).
Everest Biolabs Atlas EV and human serum albumin (HSA) ELISA kits can be used to optimize EV yield and purity during fraction collection.
| Column volume | 8.75 mL |
| Input sample volume | 0.5 - 1.0 mL |
| Sample types | Plasma Serum Urine CSF Cell culture media |
| Resin types | 4% or 6% cross-linked agarose beads |
| Exclusion limit | 35 nm or 20 nm |
| Column reproducibility (CV) | 5% |
Frequently Asked Questions
How many times can Apex columns be used?
We have tested columns for up to 5 uses.
What is the repeatability of isolation after regeneration cycles?
Apex columns have a 5% CV in EV yield for up to 5 uses when measured with the Atlas EV ELISA. We know how important repeatability is, so we developed a tightly controlled process for manufacturing columns to ensure the most repeatable columns.
Should I use Apex 6B or Apex 4B?
Apex 6B (SEC columns packed with Sepharose CL-6B resin) are optimized for the highest total EV recovery. These are ideal for targeted downstream analysis, such as immunoassays, label-based imaging assays, label-based flow assays, western blots, and pull-down using a specific surface marker. Apex 4B (SEC columns packed with Sepharose CL-4B resin) are optimized for the highest purity. These are Ideal for unbiased downstream analysis, including proteomics and RNA-seq.
